Figure 1.

Chemical composition of TX140 and its application at different anatomical sites; A) schematic representation of PNPHO along with its chemical composition and ¹H‐NMR spectrum of the polymer with the use of Thymosin‐β4 to conjugate with PNPHO. B) The region of interest, (m) peak, in ¹H‐NMR spectra of PNPHO polymer solution i) and PNPHO‐co‐Thymosin‐β4 with 30 mg mL⁻¹ at different temperatures ranging from 17 to 24 °C. D₂O/(m) of PNPHO‐co‐Thymosin‐β4 solution at different temperature to find LCST of the solutions ii). LCST of the different PNPHO‐co‐Thymosin‐β4 solution with different Thymosin‐β4 concentration (iii, source data in Figure S4 (Supporting Information), and n = 10). C) The hydrogel is injectable and forms a matrix in physiological conditions in a live sheep osteotomy model (n = 6). D) Adhesion and retention of TX140 in an ex vivo bovine cadaveric subchondral defect model without the need for physical containment in a dynamic model after 100 cycles of complete joint motion (n = 12). E) Demonstration of underwater and dynamic stability of the TX140 hydrogels applied to seal an 8 mm puncture in porcine heart tissue (n = 6). The hydrogels remained stable after 24 h soaking in a 37 °C water bath (Movies S1 and S2, Supporting Information). F) live mice subcutaneous injection model that confirms gelation and adhesion of TX140 without any physical containment (n = 24).