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Effects of Ti3C2T x on hPDLC osteogenic differentiation. a) ALP staining on day 7 and mineralized nodules stained with ARS on day 21. b) Mineralized nodule levels on day 21. c) ALP activity levels on day 7. d) Relative protein levels of the osteogenic factors were determined by western blots on day 7. e) Real‐time PCR analysis of the relative mRNA levels on day 5. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.
