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. 2023 Mar 15;12(17):2202271. doi: 10.1002/adhm.202202271

Figure 2.

Figure 2

Characterization of the KO by sequencing and protein levels reveals high editing efficiency and low residual levels of TAK1 protein. A) Sanger sequencing of WT and KO samples from 3 different donors, with percentage of off‐frame sequences per total number of alleles (n = 3). B) Sanger sequencing of KO populations for three passages after electroporation from 3 different donors (n = 3). C) Western Blot and D) quantification of TAK1 in WT and KO cells of 3 donors, normalized to GAPDH protein levels. F) Percentages of editing at the 8 most probable off‐target sites by Sanger sequencing (n = 3). Error bars represent SD. OT: off‐target.