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. 2023 Mar 15;12(17):2202271. doi: 10.1002/adhm.202202271

Figure 6.

Figure 6

TAK1‐KO cells produce high quality ECM when embedded in a hyaluronan‐based hydrogel in inflamed conditions. A) Schematic of the experiment. Rings were formed from bovine cartilage and filled with hydrogel and cells, then cultured for 6 weeks in the presence of indicated inflammatory stimuli before proceeding with the analysis. B) Bern score to assess the quality of the samples maturation (two‐way ANOVA, n = 9 from 3 donors). C,E,G) Histology of the samples by Safranin O, collagen 2 and 1 immunostaining and D,F,H), Quantification of the staining intensity (two‐way ANOVA, n = 9 from 3 donors). Bovine cartilage ring dimensions: outer diameter, 8 mm, inner diameter 4 mm; hydrogel diameter: 4 mm, 1 mm thick. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.