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Kinetics of NS1, cyclin A, p21cip1, p34cdc2, and p33cdk2 expression in FRNS1.25EJ1. The cells were treated with dexamethasone and harvested at the indicated times. Crude cell extracts (for cyclin A, NS1, and p33cdk2) or extracts purified on p9 beads (for p21cip1 and p34cdc2) were used for detection by Western blotting. Hence, the signal observed here for p21 represents the fraction of the protein which is bound to cyclin/Cdk complexes.
