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. 2022 Dec 23;12(9):2202691. doi: 10.1002/adhm.202202691

Figure 1.

Figure 1

ESC vaccination prevents bladder cancer growth. a) Scheme of immunization. Vaccines were prepared with sorting ESCs, irradiation, resuspension in adjuvant solution, and s.c. injection. b,c) In the prophylactic mouse model, Vaccination of mice with ESC vaccine resulted in a complete rejection of the cancer cells by 4 weeks and overall reductions in MB49 tumor size (n = 5 per group). Representative images showed in (b) and Quantification of the tumor size data presented in (c). Experiments were repeated three times. d,e) In the therapeutic mouse model, Vaccination of C57BL/6 mice with ESC (129) + CpG + GM‐CSF resulted in significant reduction of MB49 tumor sizes (n = 5). Representative images showed in (d) and Quantification of the tumor size data presented in (e). Experiments were repeated three times. f,g) In the therapeutic mouse model, Vaccination of C57BL/6 mice with ESC (C57) resulted in significant reduction of MB49 tumor sizes (n = 4). Representative images shown in (d) and quantification of the tumor size data was presented in (e). Experiments were repeated three times. Data in (c), (e), and (g) represent mean ± SEM (Student's t‐test ANOVA with Tukey's multiple comparison test). *p < 0.05, **p < 0.001, ***p < 0.001, ****p < 0.0001.