Figure 4.

The underlying mechanism for cancer cell capture on nanostructured surfaces with a surface roughness selective manner. a) Quantification of the relative conjugated antibodies on nanostructured surfaces with different surface roughness (n = 4–5, 3 technical replicates). The antibody‐conjugated surfaces are immunofluorescence stained by Cy5‐conjugated goat polyclonal secondary antibody to mouse IgG. b) Representative SEM images of adhered cancer cells on nanostructured surfaces and c) the filopodia length of the adhered cancer cells on nanostructured surfaces (n = 10–15, 3 technical replicates). * indicates a statistically significant comparison with P < 0.05 (one‐way ANOVA). The scale bar indicates 10 µm. d) Representative fluorescence images of paxillin immunostaining cancer cells on mPG0, mPG4, and mPG24 surfaces, respectively. The scale bar indicates 10 µm.