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. 2023 Nov 12;13(3):2300174. doi: 10.1002/adhm.202300174

Figure 2.

Figure 2

Cartilage microtissues self‐organize a cartilage‐rich ECM. A) Total levels of key cartilage ECM components; DNA, sGAG, and collagen are provided at weekly timepoints for 28 days of chondrogenic cultivation. Total levels of sGAG and collagen are significantly higher in the microtissue group by day 28. Additionally, the sGAG to collagen ratio within the engineered tissues is given. B) sGAG and collagen levels normalized to DNA show that cells within the self‐organizing microtissues produce significantly higher amounts of sGAG at day 21 and 28. C) sGAG and collagen levels normalized to wet‐weight (ww) demonstrate near native levels of sGAG are accumulated in both groups by day 28, with significantly higher levels achieved when using microtissues. However, levels of collagen remain below native levels. N = 3, significant differences were determined using an ordinary two‐way ANOVA with a Šídák's multiple comparisons test where, * denote p < 0.05, ** denotes p < 0.01, *** denotes p < 0.001, and **** denotes p < 0.0001.