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. 2022 Mar 21;11(12):2102581. doi: 10.1002/adhm.202102581

Figure 4.

Figure 4

Immunofluorescence images of the engineered 3D microvasculature. A) Fluorescence images of the engineered blood vessel (red: RFP‐expressing HUVECs; green: VE‐cadherin; blue: DAPI). i) RFP‐expressing HUVECs formed adherens junctions of VE‐cadherin. A 3D reconstructed image of the cross‐section shows the hollow lumen structure. Scale bar: 100 µm. ii) Confocal microscopic image of angiogenic sprouts grown from the main HUVEC microvasculature. Scale bar: 200 µm. iii) Enlarged view of the sprouted capillaries showing VE‐cadherin expression. Scale bar: 50 µm. B) Fluorescence images of the vascularized lung cancer model. i) Z‐projection of the 3D vascularized tumor model (red: RFP‐HUVEC and green: GFP‐A549). Scale bar: 200 µm. ii) A 3D reconstructed view of the vascularized lung cancer model (red: RFP‐HUVEC, green: GFP‐A549, and blue: DAPI). iii) Vascularized tumor system perfused with 40 kDa FITC‐labeled dextran. Scale bar: 200 µm.