Figure 7.

Impact of 0.5% w/v agarose gel on overall compound permeation. By comparing the apparent permeability coefficients (P _app) of four structurally diverse antibiotics (ciprofloxacin, tetracycline, novobiocin and rifampicin) obtained from either pure 0.5% w/v agarose gel coating or combined coating with vesicle suspensions and agarose gel, differences in the permeation velocity become noticeable. The permeation through the pure agarose gel layer is significantly faster than through the respective combinations of agarose gel and vesicle‐based coating. Columns represent mean + SD. N ≥ 9 from at least 3 independent experiments. Two‐way ANOVA with Dunnett's multiple comparisons post‐hoc analysis was performed using antibiotic‐specific P _app values of agarose as a control. **P < 0.01, ***P < 0.001, ****P < 0.0001