Table 4.
Primer sequence of the osteogenic genes investigated
| Gene | Primer sequences |
|---|---|
| GAPDH (housekeeping gene) |
5′‐CCATGGTGTCTGAGCGATGT 5′‐CGCTCTCTGCTCCTCCTGTT |
| RUNX 2 |
5′‐GGAGTGGACGAGGCAAGAGTTT 5′‐AGCTTCTGTCTGTGCCTTCTGG |
| Bone morphogenetic protein 2 (BMP2) |
5′‐GCATCTGTTCTCGGAAAACCT 5′‐ACTACCAGAAACGAGTGGGAA |
| Osteopontin (OPN) |
5′‐CCAAGTAAGTCCAACGAAAG 5′‐GGTGATGTCCTCGTCTGTA |
| Osteocalcin (OCN) |
5′‐CGCCTGGGTCTCTTCACTAC 5′‐TGAGAGCCCTCACACTCCTC |
| Collagen I (COL I) |
5′‐GATTCCCTGGACCTAAAGGTGC 5′‐AGCCTCTCCATCTTTGCCAGCA |
| Podoplanin (E11) |
5′‐GTGTAACAGGCATTCGCATCG 5′‐TGTGGCGCTTGGACTTTGT |