Figure 4.

Quantification of major extensions’ length, minor extensions’ length, and cytoskeletal areas of U251 cells. The sample sizes (n) for each of the graphs are as follows: A) Co‐culture 2D = 27, co‐culture 3D = 42, U251 2D = 93, U251 3D = 37; B) Co‐culture 2D = 53, co‐culture 3D = 52, U251 2D = 49, U251 3D = 54; C) Co‐culture 2D = 21, co‐culture 3D = 16, U251 2D = 26, U251 3D = 23. The p‐values of the measurements are indicated. *p = 0.06, **p < 0.05, ***p < 0.01, ****p < 0.001, ***** p < 0.0001.