Figure 9.
Hep‐G2 in 3D bioprinted scaffolds after bioink storage for 1 week (W1) in comparison to the unstored control group (W0), cultivated over 14 days. A) Proliferating nuclei and cell cluster formation. After 7 and 14 days of cultivation, scaffolds were incubated with EdU for 6 h before fixation. Cells that proliferated in that time frame incorporated EdU. EdU‐positive nuclei: green, counterstaining for all nuclei with DAPI: blue. B) Immunofluorescence staining for nuclei (DAPI, blue), cytoskeletons (actin, green), and albumin (red) after 14 days of cultivation. Scale bars = 50 µm for all.