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. 2021 Jul 19;33(36):2102624. doi: 10.1002/adma.202102624

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© 2021 The Authors. Advanced Materials published by Wiley‐VCH GmbH

This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.

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In vivo Matrigel plug assay of structured and non‐structured samples. A) Matrigel‐injected BALB/c‐nude mice. Structured or non‐structured spheroids encapsulated in Matrigel are injected subcutaneously. B) Aspect of the Matrigel plugs and blood vessel formation (black arrow). C) Immunohistochemical images of α‐SMA and CD31 in the Matrigel sections of structured and non‐structured spheroids. The black arrowheads indicate vessel growth inside the spheroids. D) Matrigel sections stained with H&E. E) TUNEL assay of Matrigel sections. Cell nuclei (DAPI) and condensed pyknotic nuclei (red) are merged indicating apoptotic cells (white arrowheads). Scale bars represent 100 µm.