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. 2022 Dec 18;12(7):2201748. doi: 10.1002/adhm.202201748

Figure 5.

Figure 5

Rheological properties of BSA amyloid‐based hydrogels depend on the pH used during gelation. A) Storage (Gʹ) and loss (Gʺ) moduli for 5% hydrogels prepared with 40 mm TCEP as a function of angular frequency. The frequency dependence of Gʹ for gels prepared at pH 7.4 was slightly larger for those prepared at pH 3.6. Data from three different batches and standard deviation are presented. Red lines represent log(Y) = A + B × log(X). B) Loss tangent (tanδ = Gʺ/Gʹ) as a function of angular frequency, calculated from data presented in (A). C) Storage modulus for 5% hydrogels prepared with 40 mm TCEP as a function of strain. Gels prepared at pH 7.4 showed strain softening above a strain of ≈ 5%, whereas gels prepared at pH 3.6 strain stiffening, before breaking at 30%. One of three independent experiments is shown. Inset shows magnified the region where strain stiffening is apparent. D) Stress relaxation (normalized to maximum stress) after rapid application of 10% strain for 5% hydrogels prepared with 40 mm TCEP. The mean of three independent experiments is presented; error bars (SD) are too small to be visible. Hydrogels prepared at pH 7.4 exhibited faster stress relaxation compared to those prepared at pH 3.6.