Figure 6.
Swelling behavior of BSA hydrogels depends on the pH used during preparation. A) Swelling of 5% BSA hydrogels (250 µL), prepared with 40 mm TCEP at pH 3.6 or pH 7.4, in Milli‐Q water, PBS 10 or 150 mm NaCl over time. Mean and standard deviations from three independent experiments are presented. B) The dissolution time of 5% BSA hydrogels (250 µL), prepared with 40 mm TCEP at pH 3.6 or pH 7.4, in presence of 3 or 6 m GuHCl in PBS. Each data point corresponds to an independent experiment (N = 3). Data were compared using unpaired t‐tests with Welch's correction. C) Self‐healing demonstration for a 5% BSA hydrogel prepared at pH 3.6 with 40 mm TCEP. After 24 h, the hydrogel was damaged using a syringe needle and its recovery was monitored over time. D) Two 10% BSA hydrogels, prepared at pH 3.6 with 80 mm TCEP, were cut into pieces and then placed in a Teflon mold overnight. The hydrogel pieces were joined together and formed a hydrogel that could resist force application by tweezer pulling.