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. 2021 Oct 18;10(23):2101094. doi: 10.1002/adhm.202101094

Figure 3.

Figure 3

Live/dead and histological analysis of HA‐TG and reinforced HA‐TG samples. A) Live/dead immunofluorescence was performed using a 2‐photon microscope. At day 1, cells were round in shape and sparse in number. From day 21, proliferation and spreading of cells was evident. Second harmonic generation allowed to observe collagen fiber deposition (blue). Cells continued to proliferate and spread over the course of the entire experiment. B) Histological analysis showing glycosaminoglycans (Safranin O), Collagen I and Collagen II deposition over the course of 63 d. No significant difference could be observed between the HA‐TG and the reinforced HA‐TG samples, in which cells continued to deposit matrix. The reinforced scaffold did not interfere with the deposition of matrix. C) 3D‐view of the collagen deposition by cells at day 42. D) Deposition of matrix near the reinforced structure has an even transition, with no difference with respect to the rest of the gel. Cells remain alive and unaffected by the presence of the reinforced transition zone.