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[Preprint]. 2024 Sep 24:rs.3.rs-4909396. [Version 1] doi: 10.21203/rs.3.rs-4909396/v1

Figure 3: Memory T cell recall responses against P. yoelii candidate antigens, P36 and P52.

Figure 3:

Figure 3:

Splenocytes and liver-mononuclear cells from immunized mice groups depicted in panel a of figure 2 were stimulated overnight in vitro with the P36 or P52 proteins and were tracked through flowcytometry to identify the activation of CD4+ and CD8+ memory T cells using the IFN-γ marker. a-d). Memory populations (Tcm and Tem/e) among CD4+ and CD8+ T cells of spleen (a and b) and liver (c and d) were tracked for IFN-γ production following P36 or P52 protein stimulation. A fluorescence minus one (FMO) control was used to gate on IFN-γ expressing CD8+ and CD4+ T cells (S figure 2, panel c and d). To quantify T cell activation following antigen stimulation, protein antigen was not added to unstimulated wells (UN). Frequency of T cell activation following either P36 or P52 protein stimulation was compared using the graphs on right side or at bottom of each panel using the marker IFN-γ. N=10 mice per group. Data is from two or three independent experiments. Data are the mean ± SEM. Data were analyzed by Mann-Whitney test. P<0.05 is considered significant. * P<0.05, ** P<0.01, *** P<0.001 **** P<0.0001.