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[Preprint]. 2024 Sep 28:2024.09.26.24314339. [Version 1] doi: 10.1101/2024.09.26.24314339

Genetic Risk of Reticular Pseudodrusen in Age-Related Macular Degeneration: HTRA1 /lncRNA BX842242.1 dominates, with no evidence for Complement Cascade involvement

Samaneh Farashi, Carla J Abbott, Brendan RE Ansell, Zhichao Wu, Lebriz Altay, Ella Arnon, Louis Arnould, Yelena Bagdasarova, Konstantinos Balaskas, Fred K Chen, Emily Chew, Itay Chowers, Steven Clarke, Catherine Cukras, Cécile Delcourt, Marie-Noëlle Delyfer, Anneke I den Hollander, Sascha Fauser, Robert P Finger, Pierre-Henry Gabrielle, Jiru Han, Lauren AB Hodgson, Ruth Hogg, Frank G Holz, Carel Hoyng, Himeesh Kumar, Eleonora M Lad, Aaron Lee, Ulrich FO Luhmann, Matthias M Mauschitz, Amy J McKnight, Samuel McLenachan, Aniket Mishra, Ismail Moghul, Luz D Orozco, Danuta M Sampson, Liam W Scott, Vasilena Sitnilska, Scott Song, Amy Stockwell, Anand Swaroop, Jan H Terheyden, Liran Tiosano, Adnan Tufail, Brian L Yaspan; MACUSTAR consortium; NICOLA consortium, Alice Pébay, Erica L Fletcher, Robyn H Guymer, Melanie Bahlo
PMCID: PMC11469453  PMID: 39399049

Abstract

Age-related macular degeneration (AMD) is a multifactorial retinal disease with a large genetic risk contribution. Reticular pseudodrusen (RPD) is a sub-phenotype of AMD with a high risk of progression to late vision threatening AMD. In a genome-wide association study of 2,165 AMD+/RPD+ and 4,181 AMD+/RPD-compared to 7,660 control participants, both chromosomes 1 ( CFH ) and 10 ( ARMS2/HTRA1 ) major AMD risk loci were reidentified. However association was only detected for the chromosome 10 locus when comparing AMD+/RPD+ to AMD+/RPD-cases. The chromosome 1 locus was notably absent. The chromosome 10 RPD risk region contains a long non-coding RNA (ENSG00000285955/BX842242.1) which colocalizes with genetic markers of retinal thickness. BX842242.1 has a strong retinal eQTL signal, pinpointing the parafoveal photoreceptor outer segment layer. Whole genome sequencing of phenotypically extreme RPD cases identified even stronger enrichment for the chromosome 10 risk genotype.

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