Table 2:
Description of AAN nuclei, including their primary neurotransmitter-specific cell bodies, histological staining, and corresponding locations for manual annotation.
| Brainstem arousal nucleus |
Abbreviation | ROI color |
Primary neurotransmitter |
Histology and immunohistochemistry |
|---|---|---|---|---|
| Dorsal Raphe | DR | Serotonin | Positive staining for anti-tryptophan hydroxylase | |
| Median Raphe | MnR | Serotonin | Positive staining for anti-tryptophan hydroxylase | |
| Locus Coeruleus | LC | Norepinephrine | Positive staining for tyrosine hydroxylase, and visible in H&E-LFB | |
| Laterodorsal Tegmental Nucleus | LDTg | Acetylcholine | Annotated with H&E-LFB | |
| Parabrachial Complex | PBC | Glutamate | Annotated with H&E-LFB | |
| Pontis Oralis | PnO | Glutamate | Annotated with H&E-LFB | |
| Midbrain Reticular Formation | mRt | Glutamate | Annotated with H&E-LFB | |
| Pedunculotegmental nucleus | PTg | Acetylcholine | Annotated with H&E-LFB | |
| Periaqueductal Grey | PAG | Multiple | Annotated with H&E-LFB | |
| Ventral Tegmental Area | VTA | Dopamine | Positive staining tyrosine hydroxylase |