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. 2024 Oct 11;19(10):e0310547. doi: 10.1371/journal.pone.0310547

Fig 2. GFP expression in HEK293T cells transfected with plasmids and Electropherogram of WT and mutant plasmids digested with XbaI/NotI.

Fig 2

(a) Green fluorescent protein expression after transfection of cells carrying unloaded (Control), wild-type (LDLR -WT), Gly343Ser mutant (LDLR- c.1027G>A), and Ala627Thr mutant (LDLR- 1879G>A), suggesting that there was no significant difference in transfection efficiency among the groups. (b) Electropherogram of wild-type plasmid digested with XbaI/NotI; (c)Electropherogram of mutant c.1027G>A plasmid digested with XbaI/NotI; (d) Electropherogram of mutant c. 1879G>A plasmid digested with XbaI/NotI, Lane 1: undigested electropherogram, Lane 2: electropherogram after double digestion, Lane 3: DNA marker.