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. 2024 Oct 11;19(10):e0310547. doi: 10.1371/journal.pone.0310547

Fig 4. Relative LDLR mRNA and protein expression of control, LDLR-WT, Gly343Ser mutant and Ala627Thr mutant in HEK293T cells.

Fig 4

(a) Relative LDLR mRNA expression levels (*, P < 0.05, **, P < 0.01, ***, P < 0.001) in HEK293T cells by Control, LDLR-WT, Gly343Ser mutant (LDLR- c.1027G>A), and Ala627Thr mutant (LDLR- 1879G>A). (b, c) Based on the enzyme-linked immunosorbent assay (ELISA) standard curve, the expression of Control, LDLR-WT, Gly343Ser mutant (LDLR- c.1027G>A), and Ala627Thr mutant (LDLR- 1879G>A) in the supernatant of HEK293T cells was calculated and cell lysate. (*, P < 0.05, **, P < 0.01, ***, P < 0.001) (d) Western blotting was used to detect the expression of LDLR protein in HEK293T cells by Control, LDLR-WT, Gly343Ser mutant (LDLR- c.1027G>A), and Ala627Thr mutant (LDLR- 1879G>A), respectively.