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. 2024 Oct 11;13:e85042. doi: 10.7554/eLife.85042

Figure 4. X-inactivation in Ofd1 mutant tongue.

(A–E) Frontal sections showing immunohistochemistry of GFP (A–C) and MyoD (D, E) in Wnt1Cre(HET);Hprtfl (A), Ofd1fl;Wnt1Cre(HM);Hprtfl (B), Ofd1fl/WT;Wnt1Cre(HET);Hprtfl (C), wild-type (D), and Ofd1fl/WT;Wnt1Cre(HET) (E) mice at embryonic day (E) 11. P, paternal; M, maternal. (F, G) Double immunohistochemistry of GFP and MyoD (F), and GFP and C/EBPβ (G) in Ofd1fl/WT;Wnt1Cre(HET);Hprtfl mice. (H, I) Double immunohistochemistry of GFP and MyoD (H), and GFP and UCP1 (I) on cultured YFP-expressing Ofd1 (-) cranial neural crest-derived cells (CNCC), GFP-negative mesoderm-derived cells, and GFP-negative Ofd1 (+) CNCC obtained from Ofd1fl/WT;Wnt1Cre(HET);Hprtfl mice.

Figure 4.

Figure 4—figure supplement 1. Cluster of Ofd1 mutant cells in Ofd1fl/WT;Wnt1Cre(HET) mice.

Figure 4—figure supplement 1.

Sagittal view of pharyngeal region of Wnt1Cre;Hprtfl (A) and Ofd1fl;Wnt1Cre(HET);Hprtfl (B). Arrowheads indicating cluster of GFP (-) cells.