Figure 5. Hh signal in Ofd1 mutant tongue.

(A–C’) Frontal sections showing in situ hybridization of Ptch1 in wild-type (A’), Ofd1^fl;Wnt1Cre(HM) (B’), and Ofd1^fl/WT;Wnt1Cre(HET) (C’) at embryonic day (E) 10. A, B, C, bright field (counterstained section by hematoxylin) of A’, B’, C’, respectively. A’, B’, C’, high magnification of regions outlined by boxes in A, B, C, respectively. Arrow indicating mosaic Ptch1 expression (C’). (D, E) Frontal sections showing double immunohistochemistry of GFP and Ptch1 in Wnt1Cre;Hprt^fl (D) and Ofd1^fl/WT;Wnt1Cre(HET);Hprt^fl (E) mice. Arrow indicating GFP-negative region (E). (F, G) Double immunohistochemistry of YFP and MyoD (F), and YFP and Prdm16 (G) on cultured YFP-expressing cranial neural crest-derived cells (CNCC) accompanied by YFP-negative mesoderm-derived cells obtained from Wnt1Cre;R26R^YFP mice with (lower panels) or without (upper panels) cyclopamine. (H–J) Frontal sections showing histology of wild-type (F), Smo^fl/fl;Osr2Cre (G), and Ift88^fl/fl;Osr2Cre (H) at E18.5. Arrowheads indicating ectopic sparse tissue. Scale bars: 100 µm (A-C’), 200 µm (H-J)­.

Figure 5—figure supplement 1. Adipose in Smo mutant mice.

(A–D) Frontal section showing histological images of wild-type (A, C) and Smo^fl/fl;Osr2Cre(HM) (B, D) mice. B and D were high magnifications of A and C, respectively. (E–M) Quantitative PCR (q-PCR) on mRNA isolated from ectopic sparse tissue from Smo^fl/fl;Wnt1Cre, embryonic tongue muscle (ETM), and white adipose tissue (WAT). **p<0.01.

Figure 5—figure supplement 2. Acetylated α-tubulin in cranial neural crest-derived cells (CNCC) during tongue development.

Frontal section showing immunohistochemistry of acetylated α-tubulin in YFP-expressing cells of Wnt1Cre(HET);R26R^Yfp (A) and Ofd1^fl/WT;Wnt1Cre(HET);R26R^Yfp mice (B). CNCC were confirmed by YFP expression.