FIG. 3.

Transient ACV treatment cannot block HSV-1 reactivation from latency in day 35 TG cultures. Day 35 TG single-cell suspensions were prepared and pooled; half of the TG cells were depleted of CD8⁺ T cells by immunomagnetic separation. CD8⁺ T-cell-depleted (triangles) and nondepleted (circles) TG cells were incubated in culture medium with ACV for 4 days. ACV was removed, and the cultures were incubated with medium alone for an additional 10 days. Culture supernatant fluids were sampled every other day and assayed for IFN-γ content by a standard ELISA and for infectious HSV-1 (indicating HSV-1 reactivation from latency) in a plaque assay. The data are expressed as means ± standard errors of the mean in picograms of IFN-γ per milliliter (panel A, closed symbols), percent reactivation from latency (panel A, open symbols), and PFU per culture (panel B). For CD8⁺ T-cell-depleted cultures, n = 23, and for nondepleted cultures, n = 26, except in IFN-γ determination, where n = 5. Depletion of CD8⁺ T cells significantly accelerated the reactivation rate (P < 0.05) and increased viral titer on day 10 (P < 0.05).