. 2024 Aug 3;11(10):uhae218. doi: 10.1093/hr/uhae218

Table 1.

Free amino acid content of different tissues of tea seedling during SE–S4 (mg/g, DW).

Component	CsSE	CsR_S1	CsR_S2	CsR_S3	CsR_S4	CsST_S3	CsST_S4	CsL_S4
Thea	1.419 ± 0.466^d	6.984 ± 2.637^d	90.989 ± 55.375^b	157.687 ± 20.153^a	104.319 ± 25.781^b	88.316 ± 27.308^b	69.812 ± 13.349^bc	32.008 ± 2.068^cd
Ala	0.165 ± 0.061^e	0.742 ± 0.299^a	0.470 ± 0.209^abcd	0.554 ± 0.114^abc	0.573 ± 0.043^ab	0.297 ± 0.105^bcde	0.281 ± 0.022^cde	0.203 ± 0.049^de
Val	0.102 ± 0.011^bc	0.184 ± 0.076^a	0.141 ± 0.026^ab	0.151 ± 0.020^ab	0.159 ± 0.027^ab	0.098 ± 0.018^bc	0.103 ± 0.023^bc	0.067 ± 0.006^c
Leu	0.039 ± 0.019^bc	0.152 ± 0.060^a	0.074 ± 0.059^b	0.056 ± 0.008^bc	0.061 ± 0.013^bc	0.028 ± 0.012^bc	0.033 ± 0.019^bc	0.008 ± 0.008^c
Ser	0.164 ± 0.066^e	0.358 ± 0.106^e	1.471 ± 0.957^bcd	3.531 ± 0.489^a	2.454 ± 0.650^b	1.779 ± 0.620^bc	1.408 ± 0.308^cd	0.640 ± 0.162^de
Gly	0.014 ± 0.001^c	0.054 ± 0.027^b	0.068 ± 0.021^ab	0.096 ± 0.033^a	0.063 ± 0.009^ab	0.039 ± 0.014^bc	0.038 ± 0.006^bc	0.044 ± 0.010^bc
Cys	0.042 ± 0.002^b	0.093 ± 0.094^b	0.551 ± 0.334^a	0.785 ± 0.061^a	0.473 ± 0.103^a	0.711 ± 0.212^a	0.579 ± 0.100^a	0.776 ± 0.204^a
Asp	1.274 ± 0.240^ab	1.502 ± 0.163^ab	1.503 ± 1.020^ab	1.772 ± 0.321^a	1.736 ± 0.287^a	0.569 ± 0.510^b	0.665 ± 0.316^b	0.822 ± 0.279^ab
Met	ND	0.017 ± 0.018^a	0.015 ± 0.017^a	ND	ND	0.016 ± 0.015^a	0.002 ± 0.004^a	0.015 ± 0.013^a
Thr	0.087 ± 0.022^d	0.244 ± 0.082^bcd	0.286 ± 0.170^bc	0.516 ± 0.082^a	0.435 ± 0.132^ab	0.241 ± 0.081^bcd	0.254 ± 0.102^bcd	0.098 ± 0.022^cd
Lys	0.133 ± 0.026^abc	0.150 ± 0.139^ab	0.083 ± 0.049^bc	0.151 ± 0.020^ab	0.221 ± 0.039^a	0.063 ± 0.016^bc	0.087 ± 0.009^bc	0.027 ± 0.007^c
Ile	0.008 ± 0.008^bc	0.069 ± 0.039^a	0.034 ± 0.014^bc	0.026 ± 0.002^bc	0.038 ± 0.013^ab	0.017 ± 0.006^bc	0.024 ± 0.012^bc	0.003 ± 0.005^c
Glu	1.827 ± 0.204^b	2.098 ± 1.345^b	3.157 ± 2.002^ab	4.469 ± 0.205^a	3.411 ± 0.613^ab	2.061 ± 0.603^b	2.054 ± 0.239^b	2.814 ± 0.227^ab
Gln	0.451 ± 0.246^b	1.624 ± 0.705^ab	4.145 ± 2.506^a	2.551 ± 1.975^ab	2.861 ± 2.861^ab	1.251 ± 0.827^ab	1.986 ± 1.658^ab	0.694 ± 0.661^b
Pro	0.007 ± 0.008^b	0.104 ± 0.044^a	0.042 ± 0.037^b	0.007 ± 0.006^b	0.007 ± 0.012^b	0.007 ± 0.007^b	0.003 ± 0.005^b	0.007 ± 0.010^b
Hypro	ND	0.008 ± 0.005^a	ND	ND	ND	ND	ND	ND
Orn	ND	ND	0.048 ± 0.018^a	0.137 ± 0.020^a	0.125 ± 0.045^a	0.093 ± 0.044^a	0.073 ± 0.017^a	0.082 ± 0.077^a
Cit	0.014 ± 0.012^c	0.046 ± 0.018^bc	0.166 ± 0.101^ab	0.164 ± 0.046^ab	0.284 ± 0.123^a	0.099 ± 0.047^bc	0.091 ± 0.012^bc	0.093 ± 0.009^bc
Arg	6.007 ± 1.296^b	7.084 ± 2.546^b	1.511 ± 1.260^de	4.927 ± 0.204^bc	11.819 ± 1.662^a	1.772 ± 0.280^de	3.344 ± 0.895^cd	0.496 ± 0.225^e
Phe	0.264 ± 0.060^a	0.302 ± 0.727^a	0.077 ± 0.026^b	0.049 ± 0.005^b	0.032 ± 0.009^b	0.042 ± 0.013^b	0.044 ± 0.008^b	0.102 ± 0.058^b
Tyr	ND	0.060 ± 0.032^ab	ND	0.011 ± 0.019^c	0.080 ± 0.023^a	0.008 ± 0.013^c	0.031 ± 0.027^bc	0.024 ± 0.021^bc
His	0.023 ± 0.021^c	0.193 ± 0.079^bc	0.631 ± 0.489^a	0.639 ± 0.218^a	0.579 ± 0.132^ab	0.112 ± 0.032^c	0.104 ± 0.002^c	0.027 ± 0.027^c
GABA	0.029 ± 0.004^c	1.059 ± 0.695^a	0.564 ± 0.207^abc	0.766 ± 0.107^ab	0.758 ± 0.159^ab	0.423 ± 0.098^bc	0.538 ± 0.045^abc	0.137 ± 0.035^c
Total amino acids	12.067	23.128	106.028	179.046	130.491	98.041	81.555	39.188
Theanine proportion (%)	11.76	30.20	85.82	88.07	79.94	90.08	85.60	81.68
Ethylamine	ND	0.121 ± 0.038^c	0.501 ± 0.077^ab	0.687 ± 0.097^a	0.411 ± 0.201^ab	0.489 ± 0.286^ab	0.461 ± 0.040^ab	0.362 ± 0.057^bc

CsSE, seed; CsR_S1, root during S1 stage; CsR_S2, root during S2 stage; CsR_S3, root during S3 stage; CsR_S4, root during S4 stage; CsST_S3, stem during S3 stage; CsST_S4, stem during S4 stage; CsL_S4, true leaf during S4 stage. ND, not detected. Values are means±SD, and lower-case letters denote significant differences in Duncan’s multiple extreme difference test (P < 0.05).