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. 2024 Aug 6;11(10):uhae219. doi: 10.1093/hr/uhae219

Figure 5.

Figure 5

CaPOA1 is responsive to low-temperature stress. Values are means ± SD from three independent experiments. Values with different letters above the bars are significantly different at P < 0.05. (a) Phylogenetic tree analysis of the CaPOA1 family from (Capsicum annuum), tomato (Solanum lycopersicum), tobacco (Nicotiana tabacum), corn (Zea mays), rice (Oryza sativa), arabidopsis (Arabidopsis thaliana), wheat (Triticum aestivum), and strawberry (Fragaria ananassa). (b) Relative expression levels of CaPOA1 in the roots, stems, leaves, carpopodiums, flesh, skins and seeds of WT pepper, as determined by RT–qPCR. (c) Relative expression levels of CaPOA1 in WT pepper under cold conditions (4°C). (d) Luciferase complementation imaging (LCI) of the CaPOA1 native promoter after transient expression in tobacco. (e) GUS staining of the CaPOA1 native promoter after transient expression in tobacco. The results of the control (25°C/22°C day/night) and cold (4°C for 6 h) treatment groups are compared of the CaPOA1.