Extended Data Fig. 8. Effects of PRC1 depletion on transcription of lowly expressed Polycomb targets are distinct from its activation.
(a) Gene expression analysis of Meis1 after RING1B depletion (4h IAA) and after 72h of retinoic acid treatment (72h RA). Data represents average transcript per cell numbers from single molecule RNA-FISH. Error bars represent standard deviation from n = 3 biological replicates (dots) around the average values. (b) Heatmaps representing live-cell transcription imaging of Meis1 in untreated (UNT), after RING1B depletion (IAA), and upon retinoic acid treatment (72h RA). Rows represent transcription activity trajectories of individual cells (141 in total). Data represent three biological replicates. Source numerical data are available in source data.