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. 2024 Sep 11;26(10):1700–1711. doi: 10.1038/s41556-024-01493-w

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 1 — (a) Validation that the MS2x128 array is appropriately inserted into the first intron of the corresponding gene. Top: a schematic illustrating the PCR screening strategy. Bottom: PCR results for Zic2, E2f6, and Hspg2. The experiment was repeated twice. (b) Images of intronic RNA-FISH (red) and focalized MCP-GFP signal (green) indicating that MCP-GFP accumulates at sites where intronic RNA sequences for Zic2, E2f6, and Hspg2 are identified. Nuclei are labelled with 4′,6-diamidino-2-phenylindole (DAPI, blue) and outlined with a dashed line. Representative of at least 10 images each. (c) Genomic cChIP-seq snapshots for Zic2, E2f6, Meis1, HoxA7, HoxD locus (Polycomb genes) and Hspg2 (non-Polycomb gene) illustrating signal for RING1B-AID, H2AK119ub1, SUZ12 and H3K27me3. cnRNA-seq signal before and after 4h RING1B-AID depletion is also shown. The data used is from Dobrinic et al.²¹. (d) smRNA-FISH analysis of transcript-per-cell distributions for parental (MCP-GFP expressing) and MS2x128 array-containing cell lines. Source numerical data are available in source data.

Source data