Extended Data Fig. 2. Testing heritability of transcription activity of Polycomb-targets across cell divisions.
(a) A strategy to assess the number of transcripts-per-cell for Polycomb genes between monoclonal daughter cells (grey box, left). Right: examples of smRNA-FISH images of 4-cell colonies with all cells having or all cells lacking Zic2 transcripts. This shows that the expression state of Polycomb target genes can be heritably retained across cell divisions. (b) Mean number of Polycomb gene transcripts per colony vs. colony size. Individual dots represent measurements for single monoclonal colonies. The blue dashed line represents the mean number of transcripts-per-cell in all colonies measured. Note, highly- or non-expressing colonies are still found in 4-cell colonies (2 cell divisions) indicating the respective state has been maintained across cell divisions. The data was acquired in two and three biological replicates for E2f6 and Zic2, respectively. Source numerical data and unprocessed blots are available in source data.