FIG. 1.

Infection of HFFs with HCMV AD169 induces the expression of IgG Fc-binding proteins. Cells were pulse-labeled and immunoprecipitations were performed. Lane 1, material immunoprecipitated with IgG Fc (Fc) from a lysate of mock-infected cells; lane 2, material immunoprecipitated with biotinylated IgG Fc (Fc^biotin) from a lysate of mock-infected cells; lane 3, material immunoprecipitated from a lysate of HCMV-infected cells 48 h p.i. using IgG Fc; lanes 4, 5, 6, 7, and 8, material immunoprecipitated at, respectively, 12, 24, 48, 72, and 96 h p.i. from lysates for HCMV-infected cells using Fc^biotin. The asterisk indicates a polypeptide retrieved nonspecifically and in variable amounts when using Fc^biotin. Positions of prestained molecular size standards (Gibco BRL) shown in this figure are meant to represent approximate molecular sizes only. More accurate estimates of the molecular sizes of the proteins of interest (described in the text) were determined using other molecular size standards (data not shown).