FIG. 1.

Replication kinetics of HIV-1 recombinant isolates. One thousand TCID₅₀ of each virus was used per 10⁶ PHA-stimulated PBMCs. Virus production was monitored every day by p24 antigen assay. Culture supernatants were collected every day until day 7, and p24 antigen production was monitored by enzyme-linked immunosorbent assay. Data are the means of results of three different tests. To serve as a related protease inhibitor-resistant control, Q058 insertion (Ins) and Q058 deletion (Del) isolates were cultured with the IC₅₀ of each protease inhibitor (PI) and p24 values were measured daily for 7 days.