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. 2024 Sep 15;16(9):5020–5037. doi: 10.62347/OCFT1003

Figure 7.

Figure 7

Protein expressions of oxidative stress, apoptosis, and mitochondrial damage in CLI by day 14 after CLI induction. A. Protein expression of NOX-1, * vs. other groups with different symbols (†, ‡, §), P<0.0001. B. Protein expression of NOX-4, * vs. other groups with different symbols (†, ‡, §), P<0.0001. C. The oxidized protein expression, * vs. other groups with different symbols (†, ‡, §, ¶), P<0.0001 (Note: the left and right lanes shown on the upper panel represent protein molecular weight marker and control oxidized molecular protein standard, respectively). M.W. = molecular weight; DNP = 1-3 dinitrophenylhydrazone. D. Protein expression of cleaved caspase 3 (c-Casp3), * vs. other groups with different symbols (†, ‡, §, ¶), P<0.0001. E. Protein expression of cleaved poly (ADP-ribose) polymerase (c-PARP), * vs. other groups with different symbols (†, ‡, §), P<0.0001. F. Protein expression of cytosolic cytochrome C (cyt-CytoC), * vs. other groups with different symbols (†, ‡, §, ¶), P<0.0001. G. Protein expression of mitochondrial cytochrome C (mit-CytoC), * vs. other groups with different symbols (†, ‡, §, ¶), P<0.0001. H. Protein expression of phosphorylated dynamin-1-like protein (p)-DRP-1, * vs. other groups with different symbols (†, ‡, §, ¶), P<0.0001. All statistical analyses were performed by one-way ANOVA, followed by Bonferroni multiple comparison post hoc test (n = 6 for each group). Symbols (*, †, ‡, §, ¶) indicate significance (at 0.05 level). SC = sham-operated control; EPCSW-Mel = extracorporeal shock wave (ECSW) facilitated melatonin (Mel) delivery into autologous endothelial progenitor cells (EPCs); CLI = critical limb ischemia; SNAP = S-nitroso-N-acetyl-DL-penicillamine; group 1 = SC; group 2 = CLI; group 3 = CLI + SNAP; group 4 = CLI + EPCSW-Mel; group 5 = CLI + EPCSW-Mel + SNAP.