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. 2024 Sep 15;16(9):4403–4421. doi: 10.62347/PEIQ4730

Figure 1.

Figure 1

The miniTESOS tissue clearing method renders the mandible of K14-Cre;Ai14 mice transparent. A. Experimental workflow of the miniTESOS organizational transparency process. B. Procedures of the standard miniTESOS protocol. C, D. Bright-field images of the mandible of K14-Cre;Ai14 mice at E16.5 before and after transparency using the miniTESOS method. E, F. Fluorescence images of the mandible of K14-Cre;Ai14 mice at E16.5 before and after transparency using the miniTESOS method. G. Quantification of fluorescent protein brightness before and after clearing the mandible of K14-Cre;Ai14 mice at E16.5. H, I. Bright-field images of the mandible of K14-Cre;Ai14 mice at PN14.5 before and after transparency using the miniTESOS method. J, K. Fluorescence images of the mandible of K14-Cre;Ai14 mice at PN14.5 before and after transparency using the miniTESOS method. L. Quantification of fluorescent protein brightness before and after clearing the mandible of K14-Cre;Ai14 mice at PN14.5; scale bars: 100 μm. The values are presented as the mean ± SD; **P < 0.01; n = 3 per group.