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. 2024 Oct 5;14(19):e5082. doi: 10.21769/BioProtoc.5082

Table 2. Overview of the validated fluorogens and their appropriate microscopy settings.

Fluorogen λexcmax/λemmmas Excitation laser Excitation filter Emission filter
HMBR 480/541 nm 488 nm DM405/488 BA505–605 nm
HBR-3,5DM 499/558 nm 488 nm DM405/488 BA505–605 nm
HBR-3,5DOM 516/600 nm 515 nm DM458/515 BA575–620 nm