Reagent | Final concentration | Quantity or Volume |
---|---|---|
RPMI 1640 | 500 mL | |
Fetal bovine serum | 10% | 50 mL |
Sodium pyruvate (100×) | 1× | 6 mL |
MEM Non-essential amino acid (100×) | 1× | 6 mL |
Penicillin-streptomycin (100×) | 1× | 6 mL |
Antibiotic-antimycotic (100×) | 1× | 6 mL |