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. 2024 Oct 5;14(19):e5079. doi: 10.21769/BioProtoc.5079
Reagent Final concentration Quantity or Volume
RPMI 1640 500 mL
Fetal bovine serum 10% 50 mL
Sodium pyruvate (100×) 6 mL
MEM Non-essential amino acid (100×) 6 mL
Penicillin-streptomycin (100×) 6 mL
Antibiotic-antimycotic (100×) 6 mL