| Reagent | Final concentration | Quantity or Volume |
|---|---|---|
| RPMI 1640 | 40 mL | |
| Fetal bovine serum | 20% | 10 mL |
| Sodium pyruvate (100×) | 1× | 500 µL |
| MEM Non-essential amino acid (100×) | 1× | 500 µL |
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