Reagent	Final concentration	Quantity or volume
RPMI 1640 medium, no phenol red		500 mL
Fetal bovine serum	10%	50 mL
Sodium pyruvate (100×)	1×	6 mL
MEM Non-essential amino acid (100×)	1×	6 mL
Penicillin-streptomycin (100×)	1×	6 mL
Antibiotic-antimycotic (100×)	1×	6 mL
HEPES (100 mM), pH 7.5	25 mM	1.25 mL