Fig. 5.

The nucleic acid analysis of EVs. (A) Agarose gel electrophoresis for EGFR mRNA (left) and CK19 mRNA (right) detection. (M, 500 bp DNA ladder). The sequences of the fragments of EGFR and CK19 coding region were listed in Table S10. (B) Electrophoresis characterization of DNA extracted from isolated EVs and MCF-7 cells. DNA ladder: 1 kbp. (C) Plot of the exon-wide copy-number profiles of EVs isolated with UC (top) and BAIP (bottom) using Control-FREEC. (D–E) Circos plots of DNA from PC9 EVs isolated by BAIP and UC. The outer ring is chromosome length, the second layer is SNP density line chart, and the innermost layer is Indel density histogram. (F–H) Heat maps of frequency distribution with different variant classification (F), different variant types (G) and different SNV mutation types (H) from BAIP and UC based methods, respectively.