Figure 4.

Induction of intestinal G6pc1 at P12 fails to influence the development of AgRP and POMC neural projections. (A) Schematic representation of hypothalamic nuclei that were quantified (with their corresponding bregma). Representative confocal images and quantification of (B) AgRP-immunoreactive and (C) POMC-immunoreactive fibers in P20 I.G6pc^{overexp−P12} (white lozenges) and control littermates (black lozenges) (mean ± SEM; n = 5–6). Scale bar = 100 μm. 3V: 3rd ventricle; PVHant: anterior part of the paraventricular nucleus; PVHpost: posterior part of the paraventricular nucleus; LH: lateral hypothalamic area; DMH: dorsomedial nucleus. Two-way ANOVA, followed by multiple comparisons and Sidak's post-hoc test were performed as statistical analyses.