Fig. 2.

BPD mice were constructed, and there were disorders of lung development

A. Mouse pups were exposed to normoxia (21 % O₂) or hyperoxia (85 % O₂) from the day of birth. The body size of normoxia and hyperoxia groups of mice at P3, P7, P10, and P14 was compared. H, hyperoxia; N, normoxia. B. The total number of immune cells in BALF in normoxia and hyperoxia groups of mice at P14. n = 5/group. C. Lung tissues were obtained at P3, P7, P10, and P14. D. Representative tissue sections of lungs by H&E at 21 % O₂ or 85 % O₂ at P3, P7, P10, and P14. n = 9/group. Scale bar = 50 μm. E. Assessment of lung volume according to Archimedes' principle. Lung morphometry was quantified by RAC and MLI measurements. F. PAS staining was used to evaluate and compare the degree of development between normoxia and hyperoxia at P14. n = 5/group. Scale bar = 50 μm. G. Immunofluorescence detection of SP-C and T1α in lungs of the normoxia and hyperoxia groups lung tissue at P14. n = 5/group. Scale bar = 50 μm. H.WB measured and quantified the protein levels of SP-C and T1α in lungs of the normoxia and hyperoxia groups at P14. n = 5/group. (∗P＜0.05, ∗∗∗P＜0.001).