Fig. 6.

AEC II- iNKT cells co-culture and the effect of iNKT cells on AEC II under hyperoxia 72 h

A. Giemsa staining showed the morphological characteristics of iNKT cells sorted by magnetic beads. B. Schematic representation of cell grouping and treatment. C. WB analysis of SP-C, T1α protein levels. β-Actin was used as a load Control. D. AEC II immunofluorescence staining. Red fluorescence-labeled T1α, green fluorescence-labeled SP-C, and double-stained cells indicate cells in transdifferentiated state. Scale bar = 20 μm. (∗P＜0.05, ∗∗P＜0.01, ∗∗∗P＜0.001).