Fig. 7.

iNKT cells promote AEC II ferroptosis under hyperoxia

A Heat map shows the difference in gene expression between AEC II and AEC II + iNKT cells groups. B. Heat map depicting differential expression of ferroptosis-related genes. C. The mRNA expressions of GPX4, FTH1 and PTGS2 were detected by qRT-PCR. D. The protein expressions of GPX4, FTH1 and PTGS2 were detected by WB. E. Lipid peroxidation levels were measured by C11 bodipy staining. F. The contents of Fe²⁺ in the epithelium were determined by ELISA. G. The concentration of MDA in cell lysates. H. Cell morphology was observed by transmission electron microscope. scale bar = 1 μm. (∗P＜0.05, ∗∗P＜0.01, ∗∗∗P＜0.001).