Fig. 1.

Molecular and biochemical validation of candidate ac-K280 and ac-K311 monoclonal antibodies. Representative immunoblots of QBI-293 cells transfected with wild-type 2N4R human tau or acetyl-null K280R/K311R 2N4R human tau and full length CBP. Small-scale purification of ac-tau monoclonal antibodies from hybridoma supernatants were applied to blots. A ac-K280 antibody clones. B ac-K311 antibody clones. C total acetylated lysine showing acetylation of K280R and K311R tau at other residues. D Quantification of ELISA OD450 signal from monoclonal ac-K280 and ac-K311 antibodies incubated with acetylated versus non-acetylated peptides. Peptides: Ac-K311-QIVY{Lys-Ac}PVDLSKVTSC, QIVYKPVDLSKVTSC; Ac-K280-QIIN{Lys-Ac}KLDLSNVQSC, QIINKKLDLSNVQSC