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. 2024 Oct 12;12:163. doi: 10.1186/s40478-024-01865-1

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© The Author(s) 2024

Open Access This article is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License, which permits any non-commercial use, sharing, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if you modified the licensed material. You do not have permission under this licence to share adapted material derived from this article or parts of it. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by-nc-nd/4.0/.

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Fig. 3 — Immunocytochemistry validation of ac-tau monoclonal antibodies in primary neurons. Primary mouse cortical neurons were transduced for five days with lentiviruses for empty vector control, wild-type human tau, or wild-type tau and CBP-CD-NES. A Immunocytochemistry of neurons with rabbit polyclonal ac-K280 (RFP) and small-scale purification of 19B6, 23B7, and 9G4 (GFP). B Immunocytochemistry of neurons with rabbit polyclonal ac-K311 (RFP) and small-scale purification of 15E8, 14G8, 13B12, 13H11, and 21D9 (GFP). C Immunocytochemistry of large-scale purified 19B6 and 15E8 (GFP) and polyclonal ac-K280 and ac-K311 (RFP) after lentiviral transduction with wild-type or P301L human tau and CBP-CD-NES or CBP-CD-NLS. Scale bars, 125 μm. DAPI in blue