FIG. 4.

HIV replication parameters following high-multiplicity infection of HuT-78 T cells. Cells were infected with HIV_HXB2 at 1 TCID₅₀ per cell using a centrifugally enhanced protocol. (A) P24 levels in culture supernatants were measured at various time points. (B) Comparison of the total (▴), integrated (■), and 2-LTR (×) HIV DNA forms. Data were determined by PhosphorImager quantification of bands in Fig. 3. The levels of total, integrated, and 2-LTR DNA at 26 h p.i. and total HIV DNA at 14 h p.i. are shown. The dashed line indicates one DNA copy/cell.