Fig. 1.

Establishment of cardiac failure model and identification of altered lncRNAs. A–C. Echocardiography and quantitative analysis (n = 6–7). D–E. Representative images of hematoxylin and eosin (H&E) staining (upper) and wheat germ agglutinin (WGA) staining (n = 6–7). Scale bar: 100 μm. F. Quantitative analysis of the heart weight (HW)/body weight (BW) ratio (n = 6–7). G. qPCR analysis of ANP and BNP mRNA expressions (n = 6–7). H. Heat map of the top 20 altered lncRNAs in the heart. The lncRNAs with raw intensities over 20 in the microarray sequencing report are framed in red. I. qPCR analysis of lncRNAs in the samples applied for microarray sequencing (n = 3). J. qPCR validation of LncRNA AK144717 and Gm4316 (ENSMUST00000212411) in all the mice of sham and TAC group (n = 6–7). K. qPCR analysis of LncRNA AK144717 expression in the TAC models of different time (n = 6). *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; NS means not significant between groups