Fig. 3.

AK144717 knockdown alleviated the cardiac hypertrophic response in vitro. A. qPCR analysis of AK144717 expression in NMCMs treated with 100 nM Ang II for different time (12 h, 24 h, 36 h, 48 h) (n = 3). B. qPCR analysis of AK144717 in NMCMs transfected with si-AK144717 for 24, 36 and 48 h (n = 3). C–E. qPCR analysis of AK144717, ANP and BNP mRNAs in the NMCMs treated with Ang II or si-AK144717 (n = 3). F. Representative images of immunostaining (green for α-actinin, blue for DAPI) and quantitative analysis (n = 4). Scale bar: 150 μm. G–H. Western blot analysis of p-/t-ERK1/2, p-/t-NF-κB (n = 3). *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; NS means not significant between groups