Fig. 4.

AK144717 knockdown attenuated the Ang II-induced DNA damage response. A–B. Western blot analysis of p-/t-ATM, γ-H2AX in NMCMs treated with Ang II or si-AK144717 (n = 3). C–D. Representative images of immunostaining (green for γ-H2AX, blue for DAPI) and quantification of the nuclear γ-H2AX levels in NMCMs (n = 3). Scale bar: 500 μm; E–F. qPCR analysis of AK144717, ANP and BNP expression in NMCMs (n = 3). G–H. Representative images of immunostaining (green for α-actinin, blue for DAPI) and quantitative analysis (n = 4). Scale bar: 150 μm. I. Western blot analysis of p-ERK1/2, t-ERK1/2, p-NF-κB and t-NF-κB (n = 3). *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; NS means not significant between groups