Fig. 6.

AK144717 knockdown inhibited the acetylation and translocation of HMGB1 induced by Ang II. A–B. Western blot analysis of nuclear and cytoplasmic HMGB1 in the sham and TAC group (n = 6–7). GAPDH and laminB1 were used as the internal control of the cytoplasmic and nuclear proteins respectively. C–D. Western blot analysis of nuclear and cytoplasmic HMGB1 in the NMCMs treated with Ang II for different time points (24 h, 36 h, 48 h) (n = 3). E–F. Western blot analysis of nuclear and cytoplasmic HMGB1 in the NMCMs treated with Ang II or si-AK144717 (n = 3). G. HMGB1 in the NMCM culture supernatants were measured by the mouse/rat HMGB1 ELISA kit (n = 6); H–I. Western blot analysis of nuclear and cytoplasmic HMGB1 in the mice receiving TAC surgery or AAV9-shAK144717 injection (n = 6). J–K. IP analysis of the acetylation level of lysine in HMGB1. L. Co-IP analysis of the interaction between SIRT1 and HMGB1 (n = 3). *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; NS means not significant between groups